Is a specific dye for chromatin material?

Mixtures of polychrome methylene blue-eosin Y (i.e., Giemsa stain) are widely used in biological staining. They induce a striking purple coloration of chromatin DNA (the Romanowsky-Giemsa effect), which contrasts with the blue-stained RNA-containing cytoplasm and nucleoli.

Can chromatin be dyed?

chromatin The substance of chromosomes, which includes DNA, chromosomal proteins, and chromosomal RNA. Chromatin stains strongly with basic dyes. It is thought that the chromatin is most deeply stained when it is most condensed and inactive.

Which dye is used for staining chromosomes?

The stain used for dying the chromosome is acetocarmine. This stain is a DNA-specific stain and is used when the study of different mitotic stages is required. To stain chromosomes first acetocarmine dye is prepared using the carmine.

What are the stains used to stain chromosomes and chromatin Class 11?

Carmine The basic dye, is used to stain nucleic acid and chromosomes, whichpossess negative charge on them. It gives chromosomes a pink colour thus, differentiating from other cellular organelles.

Which dye is not used to stain chromosomes?

(b) Saffanin stain is not used for staining chromosomes while Basic Fuchsin, Methylene green and Carmine are used for staining chromosomes.

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What is light colored chromatin called?

Euchromatin is a lightly packed form of chromatin (DNA, RNA, and protein) that is enriched in genes, and is often (but not always) under active transcription. Euchromatin stands in contrast to heterochromatin, which is tightly packed and less accessible for transcription. 92% of the human genome is euchromatic.

How do you stain chromatin?

Mixtures of polychrome methylene blue-eosin Y (i.e., Giemsa stain) are widely used in biological staining. They induce a striking purple coloration of chromatin DNA (the Romanowsky-Giemsa effect), which contrasts with the blue-stained RNA-containing cytoplasm and nucleoli.

Which of the following stain is used for staining of chromatin Fibre?

Chromatin fibers gather into thick shortened bodies which are then called chromosomes. Crystal violet dye is employed as a histological stain and in staining of classifying bacteria.

Which dye is used to stain mitochondria?

The Janus green is a basic dye which is also used as a vital stain in histology. This dye is used for staining the mitochondria.

Is Acetocarmine a basic dye?

What is Acetocarmine. Carmine is a basic dye prepared from an insect known as Coccus cacti. Acetocarmine is produced by mixing carmine with glacial acetic acid. It is a DNA-specific stain used for the visualization of super-coiled chromosomes during the different stages of mitosis.

Which stain is usually used to Colour chromosomes Class 11?

Gentian violet, Safranin and Giemsa stain are commonly used to colour chromosomes.

Which stain is used to stain nucleus?

Although there are various nuclear stains, Methylene blue is the most commonly used nuclear stain to observe the nucleus in the cheek cells.

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Is methylene blue used to stain chromosomes?

Carmine The basic dye, is used to stain nucleic acid and chromosomes, which possess negative charge on them. It gives chromosomes a pink colour thus, differentiating from other cellular organelles. … Methylene blue It used to stain nuclei, Golgi bodies and pectic substances.