Is used to stain chromatin material?

Mixtures of polychrome methylene blue-eosin Y (i.e., Giemsa stain) are widely used in biological staining. They induce a striking purple coloration of chromatin DNA (the Romanowsky-Giemsa effect), which contrasts with the blue-stained RNA-containing cytoplasm and nucleoli.

Which of the following stain is used for staining of chromatin fiber?

Chromatin fibers gather into thick shortened bodies which are then called chromosomes. Crystal violet dye is employed as a histological stain and in staining of classifying bacteria.

Can chromatin be stained?

chromatin The substance of chromosomes, which includes DNA, chromosomal proteins, and chromosomal RNA. Chromatin stains strongly with basic dyes. It is thought that the chromatin is most deeply stained when it is most condensed and inactive.

What is used to stain chromosomes?

The stain used for dying the chromosome is acetocarmine. This stain is a DNA-specific stain and is used when the study of different mitotic stages is required. To stain chromosomes first acetocarmine dye is prepared using the carmine.

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What color does chromatin stain?

A bluish purple line around the edge of the nucleus is the nuclear envelope/nuclear membrane. The small darkly staining granules are chromatin (chromosomes). The larger dark purple structure is the nucleolus.

What is stained chromatin?

Mixtures of polychrome methylene blue-eosin Y (i.e., Giemsa stain) are widely used in biological staining. They induce a striking purple coloration of chromatin DNA (the Romanowsky-Giemsa effect), which contrasts with the blue-stained RNA-containing cytoplasm and nucleoli.

What is stain and types of stain?

Stain – Majority of the stains used for staining bacteria are of the basic type as nucleic acid of bacterial cells attract the positive ions, e.g. methylene blue, crystal violet. Acidic stains are used for background staining. Mordant – It is a chemical that forms an insoluble complex with.

What are the stains used to stain chromosomes and chromatin Class 11?

Carmine The basic dye, is used to stain nucleic acid and chromosomes, whichpossess negative charge on them. It gives chromosomes a pink colour thus, differentiating from other cellular organelles.

What is light colored chromatin called?

Euchromatin is a lightly packed form of chromatin (DNA, RNA, and protein) that is enriched in genes, and is often (but not always) under active transcription. Euchromatin stands in contrast to heterochromatin, which is tightly packed and less accessible for transcription. 92% of the human genome is euchromatic.

Which of the following stain is not used for staining chromosomes?

(b) Saffanin stain is not used for staining chromosomes while Basic Fuchsin, Methylene green and Carmine are used for staining chromosomes.

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Which stain is used to stain nucleus?

Although there are various nuclear stains, Methylene blue is the most commonly used nuclear stain to observe the nucleus in the cheek cells.

Which is vital stain?

-Vital stain includes trypan blue, vital red, and the Janus green the latter being especially suitable for observing mitochondria. … While in supravital staining the living cells take up the stain, on the other hand in vital staining the living cells stain negatively and only the dead cells stain positively.

What is used to stain mitochondria?

Answer: (d) Janus green is used to stain mitochondria. Janus green act as an indicator and changes colour according to the amount of oxygen present. It oxidizes to blue colour in.

What is Basophilic staining?

Basophilic describes the appearance of structures seen in histological sections which take up basic dyes. The structures usually stained are those that contain negative charges, such as the phosphate backbone of DNA in the cell nucleus and ribosomes.

Why do ribosomes stain Basophilic?

What structures are stained purple (basophilic)? DNA (heterochromatin and the nucleolus) in the nucleus, and RNA in ribosomes and in the rough endoplasmic reticulum are both acidic, and so haemotoxylin binds to them and stains them purple.

Why is H and E staining used?

H and E staining helps identify different types of cells and tissues and provides important information about the pattern, shape, and structure of cells in a tissue sample. It is used to help diagnose diseases, such as cancer. Also called hematoxylin and eosin staining.

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