Which of the following stain is used for staining chromosome?

Giemsa is a visible light dye that binds to DNA through intercalation and thus, is used for chromosome staining.

Which stain is used for chromosome staining?

The stain used for dying the chromosome is acetocarmine. This stain is a DNA-specific stain and is used when the study of different mitotic stages is required.

Which of the following stain is not used for staining chromosome?

(b) Saffanin stain is not used for staining chromosomes while Basic Fuchsin, Methylene green and Carmine are used for staining chromosomes.

What are the stains used to stain chromosomes and chromatin?

Mixtures of polychrome methylene blue-eosin Y (i.e., Giemsa stain) are widely used in biological staining. They induce a striking purple coloration of chromatin DNA (the Romanowsky-Giemsa effect), which contrasts with the blue-stained RNA-containing cytoplasm and nucleoli.

How do you stain chromosomes?

Staining is responsible for the alternating dark and light bands on the chromosomes noted in Figure 11.1B. The most routinely used technique stains the metaphase chromosomes with Giemsa (after using the enzyme trypsin to digest proteins). Each chromosome pair stains with its own characteristic banding pattern.

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Which stain is used in staining the material?

Gram staining uses crystal violet to stain cell walls, iodine (as a mordant), and a fuchsin or safranin counterstain to (mark all bacteria).

Is methylene blue used to stain chromosomes?

Carmine The basic dye, is used to stain nucleic acid and chromosomes, which possess negative charge on them. It gives chromosomes a pink colour thus, differentiating from other cellular organelles. … Methylene blue It used to stain nuclei, Golgi bodies and pectic substances.

Which of the following stain is used for staining of chromatin Fibre?

Chromatin fibers gather into thick shortened bodies which are then called chromosomes. Crystal violet dye is employed as a histological stain and in staining of classifying bacteria.

Which of the following stain is used for staining blood film?

Romanowsky-Type Stains. Blood films are routinely stained with a Romanowsky-type stain (e.g., Wright or Wright-Giemsa) either manually or using an automatic slide stainer. Romanowsky-type stains are composed of a mixture of eosin and oxidized methylene blue (azure) dyes.

What is Acetocarmine stain used for?

Acetocarmine is such a stain used to stain nucleic acid inside cells. As acetocarmine specifically-stain chromosomes apart from the cytoplasm, it can be used to visualize chromosomes in mitotic studies.

Why Giemsa stain is used for chromosome observation?

It can identify chromosomal aberrations such as translocations and rearrangements. It stains the trophozoite Trichomonas vaginalis, which presents with greenish discharge and motile cells on wet prep. Giemsa stain is also a differential stain, such as when it is combined with Wright stain to form Wright-Giemsa stain.

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Which is vital stain?

-Vital stain includes trypan blue, vital red, and the Janus green the latter being especially suitable for observing mitochondria. … While in supravital staining the living cells take up the stain, on the other hand in vital staining the living cells stain negatively and only the dead cells stain positively.

Why are karyotypes stained?

G-banding, G banding or Giemsa banding is a technique used in cytogenetics to produce a visible karyotype by staining condensed chromosomes. It is useful for identifying genetic diseases through the photographic representation of the entire chromosome complement.

What are karyotypes used for?

Karyotype is a test to identify and evaluate the size, shape, and number of chromosomes in a sample of body cells. Extra or missing chromosomes, or abnormal positions of chromosome pieces, can cause problems with a person’s growth, development, and body functions.